Hu, Xuejun

Research Interests

Functional biomaterials / drug delivery: use of phage and peptide libraries to direct biodegradable nanospheres to specific cell types for targeted gene delivery
Production of recombinant proteins (including membrane proteins and glyco- and glycoengineered proteins) in E. coli, P. pastoris and cell-free systems.
Antibody-directed tumour therapy: use of molecular evolution and modelling/directed mutagenesis techniques to generate novel variants of mammalian enzymes in E. coli
Protein immobilisation studies: production and engineering (affinity maturation, increasing stability) of antibody fragments in E. coli for use in immunosensors.

Professional Qualifications

Associate Professor in Dalian University
Research Fellow, Department of Microbiology and Department of Mechanical and Biomedical Engineering National University of Ireland, Galway, Ireland
Research Scientist, School of Biological & Environmental Science, Agriculture and Food Science Center, University College Dublin (UCD), Ireland
Senior Postdoctoral Researcher, Department of Chemical and Environmental Sciences, University of Limerick(UL), Ireland
Ph.D. Bio-Engineering Department, Dalian University of Technology, China
 

Publications

1. Xuejun Hu, Sylvain Robin • Shane O’Connell • Gary Walsh • J. Gerard Wall, Engineering of a fungal β-galactosidase to remove product inhibition by galactose, Appl Microbiol Biotechnol 2010) 87:1773–1782
2. Xuejun Hu, Stefania Spada, Simon White, Sarah Hudson, Edmond Magner, and Gerard Wall. Adsorption and Activity of a Domoic Acid Binding Antibody Fragment on Mesoporous Silicates. The Journal of Physical Chemistry B, 2006, 110(37): 18703- 18709.
3. Xuejun Hu, Liam O’Hara, Simon White, Edmond Magner, Marian Kane and J. Gerard Wall. Optimisation of production of a domoic acid-binding scFv antibody fragment in Escherichia coli using molecular chaperones and functional immobilisation on a mesoporous. Protein Expression and Purification. 2007 Mar,52(1):194-201.
4. Xuejun Hu, R. O'Dwyer, and J.G. Wall, Cloning, expression and characterisation of a single-chain Fv antibody fragment against domoic acid in Escherichia coli.. J Biotechnol, 2005. 120(1): p. 38-45
5. Zhang ZC, Hu Xuejun, Yang Q, Generation of high affinity human single-chain antibody against PreS1 of hepatitis B virus from immune phage-display antibody library. Hepatobiliary Pancreat Dis Int. 2004 Feb;3(1):77-81
6. Hu Xue-Jun, Zang Zhi-chao, Bao Yong-ming, Liu Wen-zhe, Gan Qiang, AN Lijia. Production of a Functional Single-chain Fv Protein in Transgenic Tobacco Root Exudates. Biotechnology letters 2002, 24: 1531-1534,
7. Hu Xue-Jun, Zhang Zhi-chao, Yuan Xiao-dong,Bao Yong-ming, AN Lij-ia. A co-expression system based on phage and phagemid to select cognate antibody-antigen pairs in vivo. High Technol Lett. 2002, 8 (2): 5-10
8. O'Dwyer R, Razzaque R, Xuejun Hu, Hollingshead SK, Wall JG: Engineering of Cysteine Residues Leads to Improved Production of a Human Dipeptidase Enzyme in E. coli. Appl Biochem Biotechnol, 2009, Oct. 159(1):178-190
9. Zhang ZC, Hu Xue-Jun, Bao Yong-Ming, Yang Qing, An Li-jia. Production of high affinity human single-chain antibody against preS1 of Hepatitis B Virus: comparison of large naïve and in vitro immune phage displayed antibody library. Progressing in Biochemistry and Biophysics. 2002, 29 (4): 572-575
10. Hu Xue-Jun, Zhang Zhi-chao, Bao Yong-ming, Yang Qin, An Lijia. An Expeditious Method to Construct T-Vector Using Eam 1105 I Cassette. Plant Molecular Biology Reporter. June 2002, 20: 189 a-e .
11. Hu Xue-Jun, Zhang Zhi-chao, Bao Yong-ming,Yang Qing, AnnLijia. A New Series of pET-derived Vector for High-Level Expression of Multiple Copies Minor Peptide inEscherichia coli. Chin. J. Biochem. Mol. Biol. 2002, 18 (3): 287-292
12. Walter, S., J.M. Brennan, C. Arunachalam, K.I. Ansari, Xuejun Hu, M.R. Khan, F. Trognitz, B. Trognitz, G. Leonard, D. Egan, and F.M. Doohan. Components of the gene network associated with genotype-dependent response of wheat to the Fusarium mycotoxin deoxynivalenol. Funct Integr Genomics, 2008, 8:421-427.
13. Qing Yang, Hu Xue-Jun, Xu Xiao-Ming, Yuan Xiao-Dong, Su Zhi-Guo, An Li-Jia. Cloning, Expression and Purification of Gussurobin, A Thrombin-like Enzyme from the Snake Venom of Gloydius ussuriensis. Acta Biochim Biophys Sin. 2002, 34 (1): 6-10
14. Qing Yang, Hu Xue-Jun, Xu Xiao-Ming, Bao Yong-Ming, An Li-jia. Expression, Purification and Partial Characterization of Recombinant Dfibrase, a Thrombin-Like Enzyme from the Venom of Glyodius shedaoensis. Prog Biochem Biophys. 2002, 29 (3): 390-393.